Natural mixtures of persistent organic pollutants (POPs) suppress ovarian follicle development, liver vitellogenin immunostaining and hepatocyte proliferation in female zebrafish (Danio rerio).
Identifieur interne : 000200 ( Main/Exploration ); précédent : 000199; suivant : 000201Natural mixtures of persistent organic pollutants (POPs) suppress ovarian follicle development, liver vitellogenin immunostaining and hepatocyte proliferation in female zebrafish (Danio rerio).
Auteurs : Marianne Kraugerud [Norvège] ; Richard William Doughty ; Jan L. Lyche ; Vidar Berg ; Nina H. Tremoen ; Peter Alestr M ; Mona Aleksandersen ; Erik RopstadSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 1879-1514 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Danio zébré (physiologie), Femelle (MeSH), Foie (effets des médicaments et des substances chimiques), Follicule ovarique (effets des médicaments et des substances chimiques), Hépatocytes (cytologie), Hépatocytes (effets des médicaments et des substances chimiques), Polluants chimiques de l'eau (toxicité), Prolifération cellulaire (effets des médicaments et des substances chimiques), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Vitellogénines (métabolisme).
- MESH :
- cytologie : Hépatocytes.
- effets des médicaments et des substances chimiques : Foie, Follicule ovarique, Hépatocytes, Prolifération cellulaire, Régulation de l'expression des gènes.
- métabolisme : Vitellogénines.
- physiologie : Danio zébré.
- toxicité : Polluants chimiques de l'eau.
- Animaux, Femelle.
English descriptors
- KwdEn :
- Animals (MeSH), Cell Proliferation (drug effects), Female (MeSH), Gene Expression Regulation (drug effects), Hepatocytes (cytology), Hepatocytes (drug effects), Liver (drug effects), Ovarian Follicle (drug effects), Vitellogenins (metabolism), Water Pollutants, Chemical (toxicity), Zebrafish (physiology).
- MESH :
- chemical , metabolism : Vitellogenins.
- cytology : Hepatocytes.
- drug effects : Cell Proliferation, Gene Expression Regulation, Hepatocytes, Liver, Ovarian Follicle.
- physiology : Zebrafish.
- chemical , toxicity : Water Pollutants, Chemical.
- Animals, Female.
Abstract
Persistent organic pollutants such as polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and dichlorodiphenyltrichloroethane (DDT) are present in high concentrations in livers of burbot (Lota lota) in Lake Mjøsa, Norway. In order to assess effects of such pollutants on fish gonadal morphology, female zebrafish were exposed in two generations by food to mixtures of pollutants extracted from livers of burbot from Lake Mjøsa (high and low dose) and Lake Losna, which represents background pollution, and compared to a control group. Ovarian follicle counts detected a significant decrease in late vitellogenic follicle stages in fish exposed to the Losna and the high concentrations of Mjøsa mixtures in fish from the first generation. In addition, proliferation of granulosa cells, visualized by immunohistochemistry against proliferating cell nuclear antigen (PCNA), was decreased in all exposure groups in either early or late vitellogenic follicle stages compared to control. This was accompanied by increased apoptosis of granulosa cells. There was a decrease in proliferation of liver hepatocytes with exposure to both Mjøsa mixtures. In addition, immunopositivity for vitellogenin in the liver was significantly lower in the Mjøsa high group than in the control group. When analysing effects of parental exposure, fish with parents exposed to Mjøsa high mixture had significantly higher numbers of perinucleolar follicles than fish with control parents. We conclude that long-term exposure of a real-life mixture of pollutants containing high- and background levels of chemicals supress ovarian follicle development, liver vitellogenin immunostaining intensity and hepatocyte proliferation in the zebrafish model.
DOI: 10.1016/j.aquatox.2012.02.031
PubMed: 22459409
Affiliations:
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Le document en format XML
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Marianne.Kraugerud@nvh.no</nlm:affiliation><country xml:lang="fr">Norvège</country><wicri:regionArea>Dept. of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, POB 8146 Dep., 0033 Oslo</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Doughty, Richard William" sort="Doughty, Richard William" uniqKey="Doughty R" first="Richard William" last="Doughty">Richard William Doughty</name></author><author><name sortKey="Lyche, Jan L" sort="Lyche, Jan L" uniqKey="Lyche J" first="Jan L" last="Lyche">Jan L. Lyche</name></author><author><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name></author><author><name sortKey="Tremoen, Nina H" sort="Tremoen, Nina H" uniqKey="Tremoen N" first="Nina H" last="Tremoen">Nina H. Tremoen</name></author><author><name sortKey="Alestr M, Peter" sort="Alestr M, Peter" uniqKey="Alestr M P" first="Peter" last="Alestr M">Peter Alestr M</name></author><author><name sortKey="Aleksandersen, Mona" sort="Aleksandersen, Mona" uniqKey="Aleksandersen M" first="Mona" last="Aleksandersen">Mona Aleksandersen</name></author><author><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="eISSN">1879-1514</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Cell Proliferation (drug effects)</term><term>Female (MeSH)</term><term>Gene Expression Regulation (drug effects)</term><term>Hepatocytes (cytology)</term><term>Hepatocytes (drug effects)</term><term>Liver (drug effects)</term><term>Ovarian Follicle (drug effects)</term><term>Vitellogenins (metabolism)</term><term>Water Pollutants, Chemical (toxicity)</term><term>Zebrafish (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Danio zébré (physiologie)</term><term>Femelle (MeSH)</term><term>Foie (effets des médicaments et des substances chimiques)</term><term>Follicule ovarique (effets des médicaments et des substances chimiques)</term><term>Hépatocytes (cytologie)</term><term>Hépatocytes (effets des médicaments et des substances chimiques)</term><term>Polluants chimiques de l'eau (toxicité)</term><term>Prolifération cellulaire (effets des médicaments et des substances chimiques)</term><term>Régulation de l'expression des gènes (effets des médicaments et des substances chimiques)</term><term>Vitellogénines (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Vitellogenins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Hépatocytes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hepatocytes</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term><term>Gene Expression Regulation</term><term>Hepatocytes</term><term>Liver</term><term>Ovarian Follicle</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Foie</term><term>Follicule ovarique</term><term>Hépatocytes</term><term>Prolifération cellulaire</term><term>Régulation de l'expression des gènes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Vitellogénines</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Danio zébré</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Zebrafish</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Polluants chimiques de l'eau</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Persistent organic pollutants such as polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and dichlorodiphenyltrichloroethane (DDT) are present in high concentrations in livers of burbot (Lota lota) in Lake Mjøsa, Norway. In order to assess effects of such pollutants on fish gonadal morphology, female zebrafish were exposed in two generations by food to mixtures of pollutants extracted from livers of burbot from Lake Mjøsa (high and low dose) and Lake Losna, which represents background pollution, and compared to a control group. Ovarian follicle counts detected a significant decrease in late vitellogenic follicle stages in fish exposed to the Losna and the high concentrations of Mjøsa mixtures in fish from the first generation. In addition, proliferation of granulosa cells, visualized by immunohistochemistry against proliferating cell nuclear antigen (PCNA), was decreased in all exposure groups in either early or late vitellogenic follicle stages compared to control. This was accompanied by increased apoptosis of granulosa cells. There was a decrease in proliferation of liver hepatocytes with exposure to both Mjøsa mixtures. In addition, immunopositivity for vitellogenin in the liver was significantly lower in the Mjøsa high group than in the control group. When analysing effects of parental exposure, fish with parents exposed to Mjøsa high mixture had significantly higher numbers of perinucleolar follicles than fish with control parents. We conclude that long-term exposure of a real-life mixture of pollutants containing high- and background levels of chemicals supress ovarian follicle development, liver vitellogenin immunostaining intensity and hepatocyte proliferation in the zebrafish model.</div></front></TEI><affiliations><list><country><li>Norvège</li></country><region><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><noCountry><name sortKey="Aleksandersen, Mona" sort="Aleksandersen, Mona" uniqKey="Aleksandersen M" first="Mona" last="Aleksandersen">Mona Aleksandersen</name><name sortKey="Alestr M, Peter" sort="Alestr M, Peter" uniqKey="Alestr M P" first="Peter" last="Alestr M">Peter Alestr M</name><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><name sortKey="Doughty, Richard William" sort="Doughty, Richard William" uniqKey="Doughty R" first="Richard William" last="Doughty">Richard William Doughty</name><name sortKey="Lyche, Jan L" sort="Lyche, Jan L" uniqKey="Lyche J" first="Jan L" last="Lyche">Jan L. Lyche</name><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><name sortKey="Tremoen, Nina H" sort="Tremoen, Nina H" uniqKey="Tremoen N" first="Nina H" last="Tremoen">Nina H. Tremoen</name></noCountry><country name="Norvège"><region name="Østlandet"><name sortKey="Kraugerud, Marianne" sort="Kraugerud, Marianne" uniqKey="Kraugerud M" first="Marianne" last="Kraugerud">Marianne Kraugerud</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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